Practical Activities

Examining the stages of mitosis
• About 2 mm of a root tip of onion bulb is cut off and placed on a microscope slide.
• A stain e.g. aceto-orcein is added and the root tip macerated using a scapel.
• A cover slip is added and observations made.
• Different stages of mitosis can be observed.

Examining the stages of meiosis
• An unopened bud of Tradescantia is obtained
• The anther is removed and placed on a microscope slide.
• A few drops of hydrochloric acid and acetic-orcein stain are added.
• A cover slip is placed on the anther.
• Pressing the cover slip gives a thin squash, which is observed under the microscope.
• Different stages of meiosis are observed.

To observe the structure of Rhizopus
• Rhizopus grow on moist bread left under suitable temperature
• A piece of moist bread is placed on a petri¬-dish or enclosed in a plastic bag and observe daily for four days.
• Under a low power microscope the sporangia and stolons can be observed.
To examine spores on sori of ferns
• Obtain the fern plant.
• Detach a frond from the plant and observe the under-side using a hand lens to see the raised brown patches - the sori.
• Open up the sorus to observe the sporangia.

Examine insect and wind pollinated flowers
• Obtain insect pollinated flowers e.g. crotalaria, hibiscus/Ipomea, Solanum, incunum.
• Note the scent, colour and nectar guides.
• A description of the calyx, corolla, androecium and gynoecium is made.
• Obtain a wmd pollinated flower e.g,' maize, star-grass, sugar-cane, Kikuyu grass.
• Observe the glumes, spikes and spikelet.
• Examine a single floret, and identify the androecium and gynoecium.

Classifying fruits
• Obtain different fruits - oranges, mangoes, maize, castor oil, bean pod, black jack .
• Observe the fruits, classify them into succulent, dry-dehiscent or indehiscent.

Dissection of Fruits
• Obtain an orange and a mango fruit.
• Make a transverse section.
• Observe the cut surface and draw and label the parts.
• Note that the fruit is differentiated into epicarp, mesocarp and endocarp.
• Obtain a pod of a legume.
• Open up the pod and observe the exposed surface.
• Draw and label the parts.
• Note that the fruit wall is not differentiated.

Dispersal of fruits and seeds
• Obtain animal dispersal fruits, like oranges, tomatoes, black jack, sodom apple.
• Identify the way by which each is adapted to dispersal by animals.
• Obtain wind dispersed fruit/seed
e.g. Nandi flame, Jacaranda Sonchus, cotton seed, Tecoma.

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